6*His Monoclonal Antibody

Volume : 50 µL
Clone Number : 3G5H8
Aliases : 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag
Product Type : Tag/Control Antibodies
Immunogen Species :
UniProt ID :
Immunogen : HHHHHH(6x His) synthetic peptide conj µgate to KLH
Raised in : Mouse
Species Reactivity : All
Tested Applications : ELISA, WB; Recommended dilution : WB : 1 : 500-1 : 2000
Background : A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.
Clonality : Monoclonal
Isotype : IgG2a
Purification Method : >95%, Protein A purified
Conj µgate : Non-conj µgated
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : 6*His
Research Areas :